Oligonucleotide dendrimers: synthesis and use as polylabelled DNA probes
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چکیده
منابع مشابه
Oligonucleotide dendrimers: synthesis and use as polylabelled DNA probes.
Oligonucleotide dendrimers were synthesized using a novel phosphoramidite synthon, tris-2,2,2-[3-(4,4'-dimethoxytrityloxy) propyloxymethyl]ethyl- N , N -diisopropylaminocyanethoxy phosphoramidite. Label, incorporated using [gamma-32P]ATP and polynucleotide kinase, was increased in proportion to the number of 5'-ends. There was a similar increase in signal when these multiply labelled oligonucle...
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Single-stranded (ss)DNA labeled probes can be used in S1 nuclease analysis, Southern, northern blotting and in situ hybridization (1,4,7,8). They are especially useful in detecting the specific expression of genes in which both strands are transcribed. Several methods for synthesis of radioactive ssDNA probes have been described (3,6,9), for example, cloning the sequence into a suitable vector ...
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DNA dendrimers with two, three, six, nine or 27 arms were reassociated as complementary pairs in solution or with an array of complementary oligonucleotides on a solid support. In all cases, duplex stabilities were greater than those of unbranched molecules of equal length. A theoretical treatment for the process of dissociation of dendrimers explains the major properties of the complexes. The ...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1997
ISSN: 1362-4962
DOI: 10.1093/nar/25.22.4447